Bryan Johnson’s HBOT protocol.
Looking for the routine he published? Start with the exact pressure, oxygen schedule, session length, and course.
His published routine is 2 ATA for 90 minutes, five days a week.
He alternates 20 minutes of oxygen with a 5-minute air break and reports completing 60 sessions.
Bryan Johnson published this personal routine in March 2025. Use it as a reference when comparing pressure, oxygen delivery, air breaks, and session planning. Read his original Blueprint entry.
What “Bryan Johnson’s protocol” means.
These are the four details people usually mean when they ask for his HBOT routine.
- 012 ATA
His published pressure setting.
- 02Mask oxygen
100% oxygen in 20-minute blocks, with 5-minute air breaks.
- 0390 minutes
He starts at 9:00 am on the days he uses HBOT.
- 0460 sessions
Five sessions a week across the course he reported.
Build your O2genes setup around the same four decisions.
Tell us you are interested in Bryan Johnson’s protocol. We will use its published reference points to help you compare the right Hard Shell HBOT cocoon® configuration.
- 01Bring the reference
2 ATA, mask oxygen, 90-minute sessions, and a 60-session course.
- 02Choose the configuration
Hard Shell HBOT cocoon® systems operate up to 1.99 ATA with automated air-break guidance. Select the oxygen-delivery configuration with our team.
- 03Plan your routine
Match the model, capacity, room, operating support, and session plan to your wellness goals.
Nine improvements Bryan reported.
He led with lower systemic inflammation and stronger longevity markers, then listed changes across gut health, circulation, nutrients, brain-health tracking, muscle oxygen, and skin.
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01
Lower inflammation
His blood inflammation fell below what the lab can measure.
Before these 60 sessions, his inflammation marker was already among the lowest 5% of people aged 18–29. Afterward, hsCRP was too low to detect. This means the test found no measurable systemic inflammation.
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02
Stronger longevity markers
His telomeres grew about 11%, from 10.3 kb to 11.4 kb.
In adult studies, telomeres usually shorten by about 20–40 base pairs a year. His increase was about 1,100. Telomeres protect the ends of chromosomes, so their length is tracked as a marker of cellular ageing.
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03
Gut bacteria
His short-chain fatty acid level rose from low to optimal.
It rose from 22.5 to 55.8 micromol/g. He also reported that n-butyrate rose from 3.8 to 11.1 micromol/g. These compounds fuel the cells lining the colon and help support the gut barrier.
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04
Gut balance
His gut metabolic imbalance score fell from 7 to 0.
The test labels 7 as high and 0 as ideal. The score reflects beneficial compounds and enzyme activity produced by gut bacteria.
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05
New blood vessels
The signal for growing new blood vessels rose to four times his starting level.
VEGF was 300% higher, four times his starting level. This signal helps form new blood vessels, improving blood flow and oxygen delivery to tissues.
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06
Muscle oxygen
He reported that more oxygen was reaching his muscles.
Muscles use oxygen to produce energy during activity, so better oxygenation can support physical performance. He did not publish the size of the change.
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07
Vitamin D
His vitamin D rose to a little over three times his starting level.
The rise was 235%, although his supplement dose had not changed for several years. Vitamin D supports calcium absorption, bones, and normal muscle function.
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08
Brain marker
His pTAU217 level fell 28.6%, from 0.14 to 0.10 pg/mL.
Researchers use this blood marker to track tau changes associated with Alzheimer’s disease. A lower result means less of that signal was detected.
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09
Skin
He reported that his skin health improved.
He described the change as dramatic. Skin relies on blood flow for oxygen and nutrients, so improved circulation can support skin quality.
